Figure 6.
EGR1 mRNA levels, a downstream target of YB-1, following incubation of MDA-MB-231 cells for 10 h with the three putative YB-1 inhibitors at a range of concentrations from 20 µmol/L to 160 µmol/L. Data were quantitated relative to EGR1 mRNA levels in untreated cells and expressed as means ± standard error of three replicates within one experiment. For 20 µmol/L concentrations of RUS0207-A006 and JK0395-B007, the EGR1 mRNA levels are significantly lower than EGR1 mRNA levels at all higher concentrations (P < 0.05, unpaired Student׳s t-test). Results shown here are representative of two independent experiments. Note: RUS0202-G005 was used at 20 to 80 µmol/L only as it precipitates into solution at concentrations above.