Table II.
Summary of histopathologic, immunologic, and serologic testing results
| Patient no. | Histopathology | Direct immunofluorescence | Indirect immunofluorescence |
Antigen-specific serologic testing |
IgE level∗ (IU/mL) | ||
|---|---|---|---|---|---|---|---|
| Monkey esophagus basement membrane zone | Salt-split skin, epidermal binding | BP180 antibody level (U/mL) |
BP230 antibody level (U/mL) |
||||
| 1 | Eosinophilic spongiosis with tagging of eosinophils at dermal-epidermal junction (Fig 2, A) | Linear deposition of IgG and C3 (2-3+) at the BMZ (Fig 2, B and C) |
IgG: + (40) IgG4: + (160) |
IgG: + (trace 20) IgG4: + (≥20) |
− (<5.00) |
− (<5.00) |
+ (1316) |
| 2 | Mixed spongiotic, micropustular, and interface dermatitis with numerous eosinophils (Fig 2, D) | 1-2+ granular C3 deposits at dermal-epidermal junction and patchy epidermal fibrin deposition | IgG: − (weak 10) IgG4: + (80) |
IgG: − <5 IgG4: + (≥10) |
− (<5.00) |
− (<5.00) |
+ (371) |
| 3 | Subacute spongiosis and papillary dermal chronic inflammation with numerous eosinophils | Strong linear C3 and IgG at the dermal-epidermal junction | N/A | N/A | + (42.2) |
− (<5.00) |
− (80.8) |
| 4 | Acute and chronic inflammation suggestive of component of hypersensitivity reaction | N/A |
IgG: + (20) IgG4: + (80) |
IgG: + (≥20) IgG4: + (≥20) |
− (<5.00) |
− (<5.00) |
− (41.0) |
Bold indicates a positive test result.
BP180, Antibody targeting BP180 antigen (collagen XVII); BP230, antibody targeting BP230 antigen (dystonin); C3, complement protein 3; Ig, immunoglobulin; N/A, not applicable.
∗
Normal range for serum IgE was defined as 0-100 IU/mL.