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. 2019 Apr 5;9(5):1061–1077. doi: 10.1016/j.apsb.2019.03.006

Figure 8.

Fig. 8

The combination therapy against the MCF-7 tumor in nude mice. (A) Tumor growth curves of mice treated with PBS, 7pep-M-RAP, 7pep-M-PTX, Free Combi, M-Combi or 7pep-M-Combi (mean ± SD, n = 6). Arrows indicate the time for injection. **P < 0.01 vs PBS; ##P < 0.01 vs 7pep-M-RAP; ▵P < 0.05 vs 7pep-M-PTX. (B) The photo of excised tumors from different treatment groups. Scale bar = 1 cm. (C) Confocal images of TUNEL assay for apoptotic cells in tumor tissue sections from different treatment groups. DNA strand breaks were labeled with Texas red (red), and nuclei were stained with Hoechst 33258 (blue). Apoptotic cells exhibited the co-localization of these two labels. White scale bars = 100 μm. (D) TEM pictures of tumor masses with different treatments. Nucleus (yellow N), typical autophagosomes (red frames) and apoptotic body (yellow arrows) are indicated. Yellow scale bars = 1.0 μm. (E) Confocal images of LC3B aggregation (red dots) in tumor tissue sections from different treatment groups by immunofluorescence staining. Yellow scale bars = 50 μm.