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. 2019 Feb 10;9(5):1021–1034. doi: 10.1016/j.apsb.2019.02.001

Figure 3.

Fig. 3

Induction of mitotic arrest by MCC1019. (A) Flow cytometric cell cycle analysis of exponentially growing A549 cells treated with MCC1019 for 24 h in a concentration range from 10 to 40 μmol/L. The data are represented as mean ± SD of three independent experiments. (B) Western blot analysis of A549 cell lysates treated with MCC1019 for 8, 16, 20, 24, 48 or 72 h. Increased expression levels of PLK1 and cyclin B1 were seen after 24 h treatment. (C) Western blot analysis of the mitotic markers PLK1, cyclin B1 and p-HH3 after treatment with different concentrations of MCC1019 for 24 h. Data represent relative expression intensity to β-actin, Error bars are ± SD of three independent experiments. (D) Immunofluorescence analysis of A549 treated with MCC1019 and DMSO (control) and stained for α-tubulin (green) and DNA (blue). The data are represented as mean ± SD of cells undergoing mitosis.