Figure 7.

MCC1019 inhibits tumor metastasis in vivo. MCC1019 (20 and 40 mg/kg/day) suppressed the lung metastasis frequency. Lung tissue sections from MCC1019 or vehicle control-treated mice (LLC-1 bearing mice model) were stained by hematoxylin. Metastasized LLC-1 cancer cells with larger nuclei were visualized in lung tissues and captured from 6–8 animals of each group. Normal images, 10× magnifications; enlarged images, 40 × magnification. (A) The bar chart represents the number of mice that survived in each group. Number of mice with lung metastatic lesions (red area) and mice without metastatic lesion (blue area) are shown. (B) MCC1019 (20 and 40 mg/kg/day) reduced the metastatic burden area. The percentage of metastatic burden area is displayed. Each dot represents one mouse. Data represent mean ± SEM. ^**P≤0.01, Mann–Whitney t-test for comparison between vehicle and MCC1019 (20 mg/kg/day). (C) Representative H&E stained lung sections images with metastatic lesions. Infiltrating metastatic cells are detailed at higher magnification. (D) Immunohistochemical determination of BUBR1 expression in formalin-fixed, paraffin-embedded LLC-1 cancer cells. The data are represented as mean ± SD of six randomly selected sections.