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. 2019 Aug 31;9(5):923–936. doi: 10.1016/j.apsb.2019.08.007

Figure 1.

Figure 1

Mar-M induces senescence in drug resistant cells, but not in normal fibroblast cells. (A) The protein level of IL-6 in the cell culture supernatant measured using ELISA in PC3/Doc cells treatment with marchantin M (Mar-M) or doxorubicin (Doxo). (B) Detection of SA-β-gal activity in PC3/Doc cells and PC3 cells treated with Mar-M or Doxo for 1 and 5 days. (C) Proliferation of PC3/Doc cells treated with Mar-M was detected using EdU and stained with DAPI (to visualize nuclei). (D) PC3/Doc cells treated with Mar-M or Doxo for 1 and 5 days. Cell cycle analyses were performed by flow cytometry. (E) Western blot to detect the level of cell cycle proteins treated with Mar-M in PC3/Doc cells. (F) siRNA knockdown of P21 and P27 was performed in combination with Mar-M treatment and analysed the SA-β-gal activity. Bar graphs show SA-β-gal positive cells. (G) Detection of SA-β-gal activity in RWPE1 cells treated with Mar-M or Doxo for 5 days. (H) Survival rate of PC3, NHF treated with Mar-M. (I) EdU incorporation was monitored by flow cytometry in NHF cells treatment with Mar-M. (J) Representative fluorescent images of EdU staining in NHF cells. Results are representative of three independent experiments. Data are mean ± SD, *P < 0.05, **P < 0.01 and ***P < 0.001. Scale bar: 100 μm.