Figure 6.

Mar-M is potently enhancing the antitumor efficacy of doxorubicin with little cytotoxicity in chemoresistant xenografts. (A) CI plot of synergism in Mar M and doxorubicin (CI value of less than 1 denotes synergism). (B) Real-time PCR was employed to analyzed mRNA levels of IL-1α、IL-1β and IL-6 in cells exposed to chemicals (Mar-M, Doxo, LPS, Mar-M+Doxo and Mar-M+LPS). (C) Tumors were quantified using bioluminescence imaging treated with Placebo (Ctrl), Mar-M(L) (8 mg/kg), Mar-M(H) (16 mg/kg), Doxo (4 mg/kg) and Mar-M plus Doxo (4 mg/kg+2 mg/kg). Significant changes in bioluminescence intensity (Photon flux; photon/s/cm²/square root between control and experimental mice). (D) Photographs of excised tumors from five groups are shown. (E) Weights of tumors from five groups are shown. (F) Body weight from mice in different groups was recorded every 2 days. (G) Ki67 stains of tumors tissues. Ki67-positive rates in each group. Scatter plot shows the % of positively stained nuclei. (H) Biochemical analysis of liver and renal function. Data are mean ± SD, ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001. Scale bar: 100 μm.