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. 2019 May 23;9(5):937–951. doi: 10.1016/j.apsb.2019.05.001

Figure 1.

Figure 1

ARS and pan-HDACi synergistically induce cell death in solid cancer cells. (A)–(B) Effects of ARS, SAHA (A) or LBH-589 (B) and combinations of the two compounds on the cell viability of Huh-7 and Hep3B hepatocellular cancer cells, HCT116 colorectal cancer cell, A549 lung cancer cells and PANC-1 pancreatic cancer cell, as measured by MTT assay after 72 h treatment. Error bars represent SD of triplicate experiments. CI values were calculated using Compusyn software for the ARS and HDACi concentrations used in the MTT assay. (C)–(D) Representative microphotographs of Huh-7 and Hep3B cells after receiving with ARS (20 μmol/L), SAHA (2 μmol/L) (C) or LBH-589 (25 nmol/L) (D) and combination treatment for 48 h respectively. Extensive cell death, swollen cells with large bubbles commonly seen after treatment with both agents. Scale bars = 40 μm. (E)–(F) Flow cytometric analysis of Hep3B cells treated with vehicle control, ARS (20 μmol/L), SAHA (2 μmol/L) (E) or LBH-589 (25 nmol/L) (F) and the combination as indicated for 48 h. Cell death was analyzed by annexin V/PI assay.