Table 1.

Validation results for fortified bovine muscle samples (n = 63).

Analyte	Transition (m/z)	eLODb (ng g−1)	Cvalc (ng g−1)	CCβ	Relative cut-off factor (RFm)d (%)	Precisione (%)	Sensitivityf (%)
AC-262536	279.2 > 195.0	0.33	2	<Cval	69	19.1	97
Andarinea	440.2 > 150.0	0.07	2	<Cval	80	12.1	100
Bicalutamidea	429.2 > 255.0	0.09	1	<Cval	58	25.4	100
BMS-564929	306.1 > 96.0	0.26	5	<Cval	54	28.1	98
GLPG0492	390.2 > 360.2	0.18	5	<Cval	42.6	35.0	100
LGD-2226	393.1 > 241.1	0.32	2	<Cval	24.4	46.1	100
LGD-4033	337.1 > 267.2	0.04	1	<Cval	61	23.7	98
Ly2452473	375.2 > 289.2	0.08	0.5	<Cval	75	15.1	98
Ostarinea	388.1 > 118.0	0.05	1	<Cval	66	20.8	100
PF-06260414	303.1 > 168.2	0.06	2	<Cval	54	27.8	100
RAD140	394.1 > 223.1	0.19	2	<Cval	67	20.0	97
S-1a	401.1 > 261.1	0.07	1	≤Cval	93	4.5	95
S-6a	435.1 > 145.0	0.08	2	≤Cval	64	22.1	95
S-9a	417.2 > 127.0	0.19	2	≤Cval	72	17.1	95
S-23a	415.2 > 145.0	0.05	1	≤Cval	82	11.0	95

^aValues calculated response-based.

^bEstimated LOD (S/N ≥ 3).

^cScreening target concentration.

^dCalculated as percentage based on the ratio of the cut-off factor and the mean response of fortified samples.

^eCalculated as coefficient of variation (CV) of the response following fortification.

^fExpressed as percentage based on the ratio of samples detected as positive in true positive samples, following fortification.