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. 2019 Oct 15;13:437. doi: 10.3389/fncel.2019.00437

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Copyright © 2019 Cui, Reed, Crynen, Ait-Ghezala, Crawford, Shen and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis of proteins identified in proteomic analysis. (A) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from WT, WT/OVX, APP, and APP/OVX mice, with β-actin as a loading control. (B) The levels of hemoglobin α, MAP1A, neuroglobin, hemoglobin β, NBEA, STIP1, MAP6, NEFL, BASP1, FASN, and Plectin were detected in the brain lysates from APP/OVX, APP/OVX early treatment group and APP/OVX late treatment group, with β-actin as a loading control. (C) The levels of hemoglobin α and MAP1A were detected in the brain lysates from WT, WT/OVX, WT/OVX early treatment group and WT/OVX late treatment group, with β-actin as a loading control. ^∗Indicates P < 0.05 compared to APP/OVX or between the designated two groups. ^∗∗Means P < 0.01 compared to APP/OVX or designated group as indicated.