Fig. 3.

ORMDL3 affects S1P levels by regulating S1PL activity. Analysis of S1P levels in a HMC-1 and b A549 cells and of S1PL activity in c HMC-1 and d A549 cells with (sh-ORMDL3) or without ORMDL3 (sh-NC) knockdown. Analysis of e S1P levels and f S1PL activity in HMC-1 cells over-expressing Flag-ORMDL3 (insert, the expression of ORMDL3 in cells under the respective conditions). FRET analysis of the CFP–S1PL and YFP–ORMDL3 interaction in g HMC-1 and h A549 cells. *p < 0.05; N = three independent experiments