Fig. 2.

Tm-cell activation and proliferation is significantly inhibited by CD147 mAb 5A12. a, b CD147 engagement by 5A12 resulted in the downregulation of TCR-induced CD69 and CD25 expression on Tm cells from RA patients (n = 8). The expression of CD69 at 6 h and CD25 at 24 h following activation of purified CD4⁺CD45RO⁺ T cells by anti-CD3/CD28 mAbs with or without 5A12 treatments was determined. c In vitro, 5A12 inhibited Tm-cell proliferation induced via TCR engagement by anti-CD3/CD28 mAbs for 72 h, as determined by CFSE staining and FACS. d Representative data from one of three experiments are shown. Histograms were gated on the Tm population. e-h Similar experiments were performed to observe the effects of 5A12 on Tn cells. i Effects of 5A12 on phosphorylation of T-cell activation by Luminex analyses. *P < 0.05; **P < 0.01; ***P < 0.001