Fig. 4.

Downregulation of CD147 inhibits Tm-cell activation and proliferation. a FACS analyses showed the suppression of CD147 expression on Tm cells via lentiviral interference. The black lines represent the negative control memory T cells (NC), and the red lines represent the CD147 knockdown of the T cells (KD). b Knockdown of CD147 significantly inhibited Tm-cell proliferation in vitro induced via TCR engagement by anti-CD3/CD28 mAbs for 120 h, as determined by CFSE staining and FACS. c, d Knockdown of CD147 resulted in a prominent decrease of CD69 in vitro induced via TCR engagement by anti-CD3 mAb for 6 h and of CD25 via TCR engagement by anti-CD3/CD28 mAbs for 24 h on Tm cells. The corresponding statistical results for the inhibition rate and representative data are shown. **P < 0.01