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. 2019 Aug 11;6:95. doi: 10.1038/s41438-019-0177-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a and c, Clusters showing the droplet distribution after amplification of melon leaf total DNA templates at the 18th-leaf stage with specific primers and probes; intensity collected via QX200 filters suited for FAM (Ch1). Left to right: clusters showing the droplet distribution after amplification with specific probes for detecting the mitochondrial genes matR, nad9, rps1, and atp6, respectively. b and d, Clusters showing the droplet distribution after amplification of melon leaf total DNA templates at the 18th-leaf stage with the specific primers and probes; intensity collected via QX200 filters suited for HEX (Ch2). Left to right: clusters showing droplet distribution after amplification with probes for detecting the nuclear gene CmDPD1. The mitochondrial and nuclear genes detected by hydrolysis probes with different fluorophores occurred in the same reaction. e, The absolute copy number of four mitochondrial genes (nad9, rps1, matR, and atp6); the nuclear-encoded single-copy genes CmDPD1 and CmWhy2 were used as internal standards. The per-cell copy numbers of mitochondrial genes were calculated with the absolute copy number and the mean C levels