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. 2019 Aug 1;6:93. doi: 10.1038/s41438-019-0175-x

Fig. 5. Transient expression of Md-miRln20 and Md-TN1-GLS in leaves from three susceptible tissue-cultured apple varieties.

Fig. 5

a Symptoms of GD, ‘GL-3’, and ‘Greensleeves’ leaves at 3 DAI with W2. Bar = 0.5 cm. b, c Expression levels of Md-miRln20 (b) and Md-TN1-GLS (c) in leaves expressing the indicated constructs, as analyzed by RT-qPCR. d Disease incidence of leaves expressing the indicated constructs. EV, empty vector of pFGC5941; Md-miRln20 STTM, overexpression of the Md-miRln20 STTM sequence under control of the 35S CaMV promoter to silence Md-miRln20; Md-TN1-GLS OE, overexpression of Md-TN1-GLS under control of the 35S CaMV promoter. Expression values are means ± SD of three biological replicates, and statistical significance was determined by Student’s t-test: *P < 0.05 and **P < 0.01