Fig. 3.

CD70 expression is associated with T_H17 and T_H1 polarization in humans. a Quantitative PCR analysis of CD70 mRNA expression relative to 18S ribosomal RNA in T_H1, T_H2, and T_H17 differentiated lymphocytes in healthy controls (n = 9). b Expression of CD70 on CD4⁺CD45RO⁺ memory T lymphocytes and IFN-γ⁺IL-17^- T_H1, IL-4⁺IFN-γ^- T_H2 and IL-17⁺IFN-γ^- T_H17 lymphocytes in healthy controls (n≥14). c Representative immunocytofluorescent confocal images of expression of CD70 (green), IFN-γ (red), and IL-17 (red) on T_H1, T_H2, and T_H17 differentiated lymphocytes in a healthy control (images representative of n = 5). d–f Percentage of CD70⁺ lymphocytes within IFN-γ⁺IL-17^-, IFN-γ^-IL-17⁺, IL-17⁺GM-CSF⁺ T_H1 and T_H17 differentiated lymphocytes with or without the addition of TGF-β1 (control or +TGF-β1) in healthy controls (n = 8). g–l Gene and protein expression levels of specific markers under normal T_H1 and T_H17 polarizing conditions (control) or when adding neutralizing antibody against CD70 alone or against both CD27 and CD70 every day for 5 days (5 µg/ml). Analysis of the expression levels of the lymphocytes was performed on day 6. g, h Gene expression of IFN-γ and T-bet mRNA relative to 18S ribosomal RNA expression in T_H1 differentiated lymphocytes (control) or T_H1 lymphocytes treated with anti-CD70 neutralizing antibody alone or with both anti-CD27 and anti-CD70 in healthy controls (n = 6). i Percentage of IFN-γ⁺IL-17⁻ production in T_H1 differentiated lymphocytes (control) or treated with anti-CD70 neutralizing antibody alone or with both anti-CD27 and anti-CD70 in healthy controls (n = 7). j, k Gene expression of IL-17 and ROR-γt mRNA, relative to 18S ribosomal RNA expression, in T_H17 differentiated lymphocytes (control) or T_H17 lymphocytes treated with anti-CD70 neutralizing antibody alone or with both anti-CD27 and anti-CD70, in healthy controls (n = 3). i Percentage of INF-γ^-IL-17⁺ production in T_H17 differentiated lymphocytes (control) or treated with anti-CD70 neutralizing antibody alone or with both anti-CD27 and anti-CD70 in healthy controls (n = 3). Statistical testing was performed with unpaired or paired t-tests when appropriate. Data are represented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001