Figure 7.

The Combination of Reovirus and KU60019 Promotes the Endosomal Acidification and Activation of Cathepsin B

(A and B) The acidification of endosomes and lysosomes was determined by LysoTracker red staining. CMeC1 cells were treated with reovirus (MOI 100) and KU60019 (2.5 μM) for 24 h before staining. (A) Histogram overlay of LysoTracker red staining of each sample is shown for representative data from one of four independent experiments. Relative median fluorescence intensity (MFI) values relative to mock treatment are indicated. Mean + SD are shown from four independent experiments. Tukey-Kramer test, *p < 0.05, **p < 0.01. (B) Representative images of samples from each treatment groups are visualized using confocal microscopy at a 100× magnification. Nuclei were stained with DAPI. Scale bars, 10 μm. (C) The enzyme activity of cathepsin B in CMeC1 cells treated as described above was measured and normalized to the value obtained with mock treatment. Mean + SD are shown from five independent experiments. Tukey-Kramer test, *p < 0.05.