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. 2019 Sep 1;6:101. doi: 10.1038/s41438-019-0184-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Expression of the candidate genes was analyzed in three individual field grown genotypes from the three groups, Psa-RMR (73, 74, 76), Psa-FR (77, 78, 79) and Psa-Sus (82, 83, 84), using real-time quantitative reverse transcription polymerase chain reaction (RT-qRT-PCR). Data represents the mean of relative gene expression of the candidate genes in three replicates of each genotype, after normalizing to the global mean expression of the two-housekeeping genes, Actin and Ubiquitin, within each replicate. Asterisks represent statistically significant differences in the mean relative expression of the candidate genes in genotypes of Psa-RMR and Psa-FR compared with the mean relative expression of genes in Psa-Sus genotypes using Student’s t-test