Table 2.
Real values of the independent variables and observed responses to the second factorial design.
| Runa | Independent variables (real values) |
Responses |
|||
|---|---|---|---|---|---|
| Ethanol (%) | Propolis-to-solvent ratio(w/v)* | YTPC (mg GAE.g−1)b | YORAC (µmol TEAC.g−1)c | YABTS (µmol TEAC.g−1)d | |
| 1 | 14.4 | 1:24.3 | 132.9 ± 0.7 | 5003.7 ± 163.5 | 2665.5 ± 13.7 |
| 2 | 84.6 | 1:24.3 | 646.5 ± 2.0 | 4925.6 ± 109.4 | 2901.9 ± 24.0 |
| 3 | 14.4 | 1:45.6 | 125.3 ± 1.0 | 3439.1 ± 2.2 | 1155.3 ± 8.1 |
| 4 | 84.6 | 1:45.6 | 651.0 ± 4.9 | 8107.4 ± 112.0 | 4689.0 ± 32.2 |
| 5 | 0 | 1:35 | 57.9 ± 1.3 | 21.3 ± 102.9 | 408.6 ± 2.4 |
| 6 | 99 | 1:35 | 1614.8 ± 28.2 | 13244.5 ± 62.8 | 13412.1 ± 49.4 |
| 7 | 49.5 | 1:20 | 278.6 ± 0.9 | 3551.8 ± 27.8 | 1219.3 ± 40.3 |
| 8 | 49.5 | 1:50 | 212.3 ± 0.5 | 2569.1 ± 177.4 | 1439.5 ± 3.4 |
| 9 | 49.5 | 1:35 | 719.4 ± 3.8 | 7605.7 ± 152.9 | 2417.4 ± 2.4 |
| 10 | 49.5 | 1:35 | 719.0 ± 1.9 | 7650.6 ± 110.9 | 2417.4 ± 7.3 |
| 11 | 49.5 | 1:35 | 719.0 ± 3.8 | 8464.1 ± 113.4 | 2574.0 ± 43.8 |
| 12 | 49.5 | 1:35 | 718.5 ± 3.8 | 6702.4 ± 76.8 | 2260.7 ± 55.60 |
The results were expressed as mean ± standard deviation.
a
Tests performed in random order.
b
Total Phenolic Content.
c
Total antioxidant capacity by ORAC.
d
Total antioxidant capacity by ABTS.
*
All assays were performed with a total volume of 30 mL and the extraction time was maintained in 20 min.