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. 2019 Sep 1;20(12):1416–1429. doi: 10.1080/15384047.2019.1647049

Figure 2.

Figure 2.

Depletion of UBE4B reveals an inhibitory effect of Cetuximab on neuroblastoma cell proliferation in vitro. (a) Two different shRNAs (shRNA1, shRNA2) were delivered to SK-N-AS neuroblastoma cells using lentiviruses resulting in undetectable levels of UBE4B. (b) Depletion of UBE4B resulted in a concomitant increase in EGFR as previously described (Sirisaengtaksin et. al. 2014). (c) Proliferation of parental SK-N-AS cells (blue circles) or those infected with lentivirus encoding a scrambled shRNA (red squares) or an shRNA targeting UBE4B (green triangles) was similar. However, Cetuximab significantly inhibited proliferation only when UBE4B was depleted (d) compared to parental cells and those infected with a scrambled shRNA measured using an MTT assay (n = 3). Graphs (b-d) show the mean ±/- S.E.M. from three independent trials. Comparisons were made using ANOVA with post hoc Dunnett multiple comparison test. * denotes p < .05, ** denotes p < .01, *** denotes p < .001.