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. 2019 Aug 7;20(12):1453–1461. doi: 10.1080/15384047.2019.1647058

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Figure 4. — LINC01133 up-regulated the target gene of miR-4784, AHDC1. (a) Bioinformatic analysis of five potential downstream genes of miR-4784. (b) The expression of AHDC1 was detected in normal cervical cells and CC cells. (c) The predicated binding sites between AHDC1 and miR-4784. (d) The expression level of LINC01133 post-transfection of pcDNA3.1/LINC01133 into ME-180 and MS751 cells was tested. (e) The luciferase activity of AHDC1-WT reporter was monitored after transfection with specific plasmids. (f) RIP assay consolidated the binding between AHDC1 and miR-4784. (g-h) qRT-PCR and western blot disclosed the effect of LINC01133 or miR-4784 on AHDC1 mRNA and protein levels. *P < .05, **P < .01, *** P < .001.