Figure 5.

Simultaneous Liver and Muscle Expression of hSGCA-SIINFEKL Increases Liver Intraparenchymal T Regulatory Cells and Exhausted CD8 T Cells

Non-parenchymal cells extracted from livers of mice treated as described in Figure 4 were analyzed by flow cytometry. (A and B) Flow cytometry histograms representing the CD4⁺FoxP3⁺ population gated on CD4⁺ cells. The histogram shows quantification. (C and D) Flow cytometry dot plots representing the CD8⁺PD1⁺ population gated on CD8⁺ cells (C). The histogram shows the quantification of the dot plots (D). (E and F) Flow cytometry dot plots representing the CD8⁺PD1⁺LAG3⁺ population gated on CD8⁺ cells (E). The histogram shows the quantification of the dot plots (F). (G and H) Flow cytometry dot plots representing the CD8⁺PD1⁺TIM3⁺ population gated on CD8⁺ cells (G). The histogram shows the quantification of the dot plots (H). In (C), (E), and (G), the blue dots represent SIINFEKL-specific MHC class I dextramer-stained T cells. Data were expressed as mean ± SD. Statistical analyses were performed by ANOVA (*p < 0.05 versus Control; n = 3 per group).