Fig. 2.

T cells from mice with induced SLE are Th1-biased and produce IFN-γ. Splenocytes from β2GPI/LPS-immunized mice (C57BL/6 [A]) or C3H/HeN [B]) were cultured with human β2GPI (15 µg/ml), IL-2 (10 units/ml), and IL-7 (10 ng/ml) for 7 days. Cells were then stimulated with PMA/ionomycin for 3 h in the presence of monensin and stained for surface and intracellular markers. Flow cytometry plots depict CD4 vs. CD8 populations, followed by IL-4, IFN-γ, and IL-17a staining for live CD4⁺ cells. All cytokine data shown were gated on 3 × 10⁴ live CD4⁺ T cells. Bar graphs summarizing the flow cytometry data show pooled data ± SE of two independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001)