Fig. 5.

Progression of SLE autoantibody production in induced and spontaneous SLE. Top panel: sera from β2GPI/LPS-immunized C3H/HeN mice were tested for IgG anti-β2GPI, anti-CL, and anti-dsDNA autoantibodies by ELISA 14 days after each immunization. As autoantibody titers increased over time, sera were evaluated at multiple dilutions. The dilution shown for each autoantibody and bleed is indicated in square brackets: anti-β2GPI (first immunization [1/100], second immunization [1/3000], and third immunization [1/5000]); anti-CL (first immunization [1/100], second and third immunizations [1/5000]); and anti-dsDNA (all immunizations [1/100]). Bottom panel: sera from MRL/lpr mice were obtained every 2 weeks, starting from 4 weeks until 10 weeks of age, and were tested for anti-β2GPI (1/100 dilution), anti-CL (1/1000 dilution), and anti-dsDNA (1/1000 dilution). Each dot represents the mean IgG antibody binding of duplicate samples for an individual mouse (n = 4–6 mice/group), and bars indicate the mean blanked OD₄₀₅ ± SE for each group