Fig. 4.

Treatment with H6F resulted in loss of mIns1B_5–14 autoreactive CD8⁺ T cell responses. a Representative statistical analysis of the average number of IFN-γ-positive spots derived from splenic CD8⁺ T cell responses to control peptide or mIns1B_5-14 within a treatment group (n = 5) are shown. The data are expressed as the mean ± SEM. Significance was determined by a paired t-test. b A representative image of IFN-γ ELISPOT assay for monitoring mIns1B_5-14-specific splenic CD8⁺ T cell responses in each peptide-treated group of nondiabetic NOD.β2m^null.HHD mice at the age of 12 weeks. The average number of IFN-γ-positive spots per 2 × 10⁵ splenic CD8⁺ T cells in triplicate cultures was calculated. T2 cells pulsed with mIns1B_5-14 or control peptide (50 µg/ml) were used as stimulators