Fig. 6.

Phenotypic characterization of CD8⁺CD25⁺ T cells in the NOD.β2m^null.HHD mice. CD8⁺CD25⁺ and CD8⁺CD25⁻ T cells were freshly isolated from the spleens of H6F-treated nondiabetic NOD.β2m^null.HHD mice at the age of 12 weeks (n = 5) and these cells were phenotyped by flow cytometry analysis. a Representative flow cytometry phenotypic profiles of CD8⁺CD25⁺ and CD8⁺CD25^- T cells in H6F-treated NOD.β2m^null.HHD mice. The numbers above the line indicate the event numbers of cells with a positive phenotype in the indicated cell subsets, and the numbers below the line indicate the total numbers of the indicated CD8⁺CD25⁺ T cells or CD8⁺CD25^- T cells. b The mean percentages of Foxp3⁺, CTLA-4⁺, CD62L⁺, CD103⁺, IFN-γ⁺ and IL-17A⁺ in CD8⁺CD25⁺ (open bars) or CD8⁺CD25^- (gray bars) T cells from H6F-treated NOD.β2m^null.HHD mice (n = 5). ***Compared to the same phenotype or cytokine production of CD8⁺CD25⁺ T cells, P < 0.001. The data are expressed as the mean ± SD. c The mean percentages of Foxp3⁺, CTLA-4⁺, CD103⁺, and CD62L⁺ in splenic CD8⁺CD25⁺ T cells or d the mean percentages of IFN-γ⁺ and IL-17A⁺ in splenic CD8⁺CD25^- T cells isolated from different peptide-treated NOD.β2m^null.HHD mice (n = 5). The data are expressed as the mean ± SD. Significance was determined by an unpaired t-test