Table 1.
Subcellular distribution of selected metabolites of ‘Braeburn’ apple fruit harvested at three distinct growth stages (30, 93, and 149 d after full bloom)
| Metabolites | Subcellular distribution [%] | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 30 d after full bloom | 93 d after full bloom | 149 d after full bloom | |||||||
| Plastid | Cytosol | Vacuole | Plastid | Cytosol | Vacuole | Plastid | Cytosol | Vacuole | |
| Carbohydrates | |||||||||
| Sucrose | 6 | 6 | 88 | 0 | 9 | 91 | 9 | 14 | 77 |
| Sorbitol | 7 | 1 | 92 | 0 | 7 | 93 | 0 | 7 | 93 |
| Fructose | 0 | 0 | 100 | 0 | 3 | 97 | 13 | 12 | 75 |
| Glucose | 0 | 0 | 100 | 0 | 3 | 97 | 0 | 6 | 94 |
| Myo-inositol | 18 | 2 | 80 | 8 | 11 | 81 | 12 | 13 | 75 |
| G3P | 26 | 11 | 63 | 55 | 19 | 26 | 16 | 33 | 51 |
| G6P | 27 | 5 | 68 | 13 | 87 | 0 | 20 | 70 | 11 |
| Organic acids | |||||||||
| Pyruvate | 24 | 1 | 75 | 9 | 15 | 76 | 28 | 14 | 58 |
| Citrate | 32 | 5 | 63 | 0 | 26 | 74 | 0 | 12 | 88 |
| Succinate | 10 | 0 | 90 | 11 | 4 | 85 | 5 | 4 | 92 |
| Fumarate | 5 | 3 | 92 | 20 | 0 | 80 | 16 | 0 | 84 |
| Malate | 0 | 0 | 100 | 0 | 4 | 96 | 0 | 4 | 96 |
| Amino acids | |||||||||
| Alanine | 10 | 0 | 90 | 26 | 12 | 62 | 0 | 14 | 86 |
| Asparagine | 14 | 0 | 85 | 7 | 3 | 90 | 23 | 1 | 77 |
| Aspartate | 1 | 0 | 99 | 5 | 6 | 89 | 7 | 0 | 93 |
| Beta-alanine | 10 | 16 | 74 | 7 | 43 | 51 | 0 | 26 | 74 |
| GABA | 5 | 0 | 95 | 27 | 1 | 71 | 16 | 0 | 84 |
| Glutamate | 25 | 0 | 75 | 0 | 33 | 67 | 0 | 0 | 100 |
| Glycine | 7 | 27 | 67 | 3 | 21 | 76 | 10 | 15 | 75 |
| Isoleucine | 9 | 57 | 34 | 0 | 100 | 0 | 6 | 92 | 2 |
| Leucine | 14 | 2 | 84 | 27 | 2 | 71 | 0 | 17 | 83 |
| Phenylalanine | 13 | 55 | 33 | 0 | 100 | 0 | 6 | 91 | 3 |
| Proline | 10 | 2 | 89 | 18 | 14 | 68 | 5 | 21 | 74 |
| Serine | 10 | 0 | 90 | 2 | 1 | 97 | 4 | 12 | 84 |
| Valine | 7 | 46 | 47 | 5 | 95 | 0 | 9 | 88 | 4 |
Metabolite levels are expressed as a percentage in the different subcellular compartments