Figure 1.
LV Production and Clarification
(A) Optimization of LV production. In total 7.9 × 107, 1.29 × 108, and 2.37 × 108 WinPac-RD cells were seeded in one HYPERFlask in harvest A, harvest B, and harvest C, respectively. SDs for harvests A and B represent duplicates and triplicates, while in harvest C all data points are all triplicates. (B) The effect of defrosting and clarification (low-speed centrifugation and filtration with Millex HP PES Express 0.45 μm) on infectivity of LV batch from harvest A (7 dps). At collection, n = 3; after defrosting and clarification, n = 12. (C) Infectivity recovery after defrosting and clarification (low-speed centrifugation and filtration with Millex HP PES Express 0.45 μm) in comparison to infectivity at harvest. Batches from harvest B were tested in defrosting experiments (n = 4) and batches from harvests B and C were tested in low-speed centrifugation (n = 14) and filtration with Millex HP PES Express 0.45 μm (n = 12) experiments. There are no statistically significant differences between samples obtained after defrosting and clarification compared to samples taken at harvest. Statistical analyses were performed using one-way ANOVA. *p < 0.05. Error bars represent mean ± 1 SD.