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Final Scale-Up LV Purification Using Nanofiber IEX
(A) Overlapping chromatograms of 400 mL LCM (harvest C, 7 and 9 dps) directly loaded onto a 0.1 mL RCQ nanofiber adsorbent module, (B) the elution profile, corresponding (C) SDS-PAGE visualized by silver staining, and (D) p24 western blot analysis of selected fractions from run II. (E) TEM image of elution fraction 5 (E5) from run II. L, load; FT, flow through; E, elution fraction.
