Figure 3.

Higher-Power Images of ELF 97 Fluorescent AP Substrate Staining of Representative Tibial Fracture Samples

Images were collected from groups showing the highest transduction in the callus. Notably, negligible tdTomato expression was seen in osteocytes of the old cortical bone (B). ELF 97 labeled osteoblasts co-labeling with tdTomato (yellow) were present in the fracture callus (C; arrows). ELF-97-negative cells that were tdTomato+ putative osteocytes were present in the callus, surrounded by new bone matrix (C; circles). Scale bars represent 100 μm.