Fig. 2. PCR detection revealed targeted deletions of AP1, SVP, and TFL1 in T1 plants.

a Map of three floral genes targeted for deletion using a pair of sgRNAs each. sgRNA sites are shown as black lines. Arrowheads represent the primers used to detect the deletions. b Lengths of PCR products from wild-type loci and the targeted deletions of AP1, SVP, and TFL1. c DNA sequences of representative deletion products. The sequence of the unmodified locus for each of the three genes is shown on the top. sgRNA target sites are underlined, and PAM sequences are in bold. Cleavage sites are shown as black arrowheads. Deleted sequences are shown with dots