Fig. 6.

PRMT6 binds and sequesters IRF3 to disrupt assembly of the TBK1–IRF3 signaling complex. a Co-immunoprecipitation and immunoblot analysis of HEK293T cells transfected with PRMT6–V5 and Myc-tagged TBK1, IKKε, IRF3, or IRF7. b Co-immunoprecipitation and immunoblot analysis of HEK293T cells transfected with PRMT6–V5 and IRF3-Flag. c Co-immunoprecipitation and immunoblot analysis of BMDMs infected with VSV for the indicated lengths of time. d Co-immunoprecipitation and immunoblot analysis of HEK293T cells transfected with IRF3-Flag, TBK1–Myc, and PRMT6 or PRMT6(dead). e Co-immunoprecipitation and immunoblot analysis of BMDMs infected with VSV for the indicated lengths of time. Data in a–e are representative of three independent experiments