Figure 1.

Cell Viability of BT4C Rat Glioma Cells after GCV Treatment Measured at Days 3 and 6 after LV-TK Transduction, and the Efficacy of LV-TK/GCV in a Rat Malignant Glioma Model

(A and B) BT4C cells were either non-transduced or transduced with treatment vector. Cell viability was analyzed with MTS assay 3 days (A) and 6 days (B) post-transduction. Experiments were carried out with three or more replicates. (C) BT4C tumor growth was monitored by MRI. Images represent the average tumor size of the specific time point. (D) Group average tumor volumes in pixels as measured from the MRI images. (E) Survival of the LV-TK treated rats bearing malignant gliomas. NT, non-transduced; MOI, multiplicity of infection; LV-TK, lentivirus vector expressing herpes simplex virus thymidine kinase; GCV, ganciclovir. Results are expressed as mean ± SEM, *p > 0.05, **p > 0.01, ***p > 0.001.