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. 2019 Oct 22;14(10):e0223689. doi: 10.1371/journal.pone.0223689

Fig 5. Cell-to-cell marker protein expression in the 2D and 3D cell culture environment.

Fig 5

(A), (B) Induction of release of the epithelial-mesenchymal transition (EMT) mechanism protein in bladder cancer cells treated with TGF-β1. After 72 h, the supernatant of the 5637 and T24 bladder cancer cell culture media was separated, and e-cadherin and n-cadherin were measured by sandwich ELISA. * p < 0.05, ** p < 0.01, ratios were compared between the 2D- and 3D-cultured TGF-β1-untreated group and the 2D- and 3D-cultured TGF-β1-treated group. Data are the mean ± SEM of three independent experiments.