Fig 5. Replication dynamics and interaction between the clone-derived DWV isolates.

(A) Average DWV RNA copies per honey bee pupa ± SD. Individual pupae copy numbers indicated by black dots. (B) Proportion of DWV reads in NGS libraries for individual, mixed, and control infections. Significant and ns differences are indicated. (C) Recombination breakpoints generated in mixed clone-derived infections. Black diamonds show the proportion of recombinant reads in individual mixed infections (left y-axis). Blue bars show average proportion of recombinant reads in a genome section (right y-axis). DWV genetic map is shown below; numerical values underlying the summary graphs are provided in S1 Data. dpi, days postinjection; DWV, deformed wing virus; extr, virus extract; LP, leader protein; NGS, next-generation sequencing; ns, nonsignificant; p.i. postinjection; WT, wild-type.