Figure 3.

Mouse liver tissue was extracted and subjected to ¹H NMR. Multivariate data analysis was performed to evaluate the differences between DMSO and TCPOBOP treated WT mice at 48 (A) and 72 h (B) time points as well as CARKO mice at 48 (C) and 72 h (D). OPLS-DA scores (left) and coefficient-coded loadings plots (right) for the models were obtained from NMR data. The OPLS-DA models were validated using a 7-fold cross validation method, and the quality of the model was described by the parameters R²X and Q² values. Color-coded correlation coefficient loading plots were employed to indicate the significance of the metabolite contribution to the class separation with a “hot” color (e.g., red) being more significant than a “cold” color (e.g., blue). A cutoff value of |r| > 0.707 (r > +0.707 and r < −0.707) was chosen for the correlation coefficient as significant based on the discrimination significance (p ≤ 0.05).