FIG 4.

Magnitude of the shift among the observed C_T values (ΔC_T) of PMA-qPCR (viable-cell-equivalent count) compared to those of qPCR (total cell equivalent count) of an overnight culture in CAMHB (A) and a 56-day-old culture on CBA plate of C. coli that were subjected to three inactivation methods (1.5% H₂O₂ and incubation at 42°C for 50 min, lethal heat treatment at 75°C for 30 min, lethal heat treatment at 95°C for 5 min) (B). Each bar represents the mean ± SD of results from three independent repetitions (triplicate aliquots).