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. 2019 Oct 1;85(20):e01518-19. doi: 10.1128/AEM.01518-19

TABLE 2.

Sequence variation statistics of the partial sequences of four housekeeping genes from Xanthomonas translucens strains isolated in Iran in comparison to those of the strains isolated in six other countries

Region, X. translucens pathovar Gene No. of
Haplotype frequencya Total no. of segregating sites % of polymorphic sites Nucleotide diversity (π) No. of mutations (η) Haplotype (gene) diversity Value for indicated neutrality test
Minimum no. of recombination events
Strains Nucleotides Haplotypes Tajima’s D Fu and Li’s D* Fu and Li’s F*
Iran
    undulosa dnaK 58b 762 8 0.137 5 0.656 0.118 × 10−2 5 0.655 −0.391 NSc 1.083 NS 0.724 NS 2
fyuA 58 522 1 0.017 0 0.000 0.000 0 0.000 NPd NP NP 0
gyrB 58 505 4 0.068 3 0.594 0.100 × 10−2 3 0.456 −0.442 NS −1.782 NS −1.602 NS 0
rpoD 58 674 7 0.120 6 0.890 0.123 × 10−2 7 0.257 −1.165 NS −0.369 NS −0.737 NS 2
    translucens dnaK 8 762 7 0.875 4 0.524 0.230 × 10−2 4 0.964 0.586 NS 0.568 NS 0.629 NS 1
fyuA 8 522 2 0.250 3 0.574 0.308 × 10−2 3 0.536 1.600 NS 1.233 NS 1.445 NS 0
gyrB 8 505 3 0.375 2 0.396 0.099 × 10−2 2 0.464 −1.310 NS −1.409 NS −1.513 NS 0
rpoD 8 674 4 0.500 7 1.038 0.540 × 10−2 7 0.786 1.662 NS 0.973 NS 1.252 NS 0
Non-Iran
    undulosa dnaK 75 762 8 0.106 8 1.049 0.129 × 10−2 8 0.598 −1.011 NS 0.512 NS 0.004 NS 1
fyuA 75 522 2 0.026 1 0.191 0.005 × 10−2 1 0.027 −1.062 NS −1.947 NS −1.958 NS 0
gyrB 75 505 2 0.026 1 0.198 0.010 × 10−2 1 0.053 −0.904 NS 0.513 NS 0.110 NS 0
rpoD 74e 674 5 0.067 5 0.741 0.031 × 10−2 5 0.155 −1.780 NS −3.024e (P < 0.05) −3.084e (P < 0.05) 0
    translucens dnaK 36e 762 2 0.055 1 0.131 0.042 × 10−2 1 0.322 0.495 NS 0.574 NS 0.636 NS 0
fyuA 37 522 2 0.054 3 0.574 0.200 × 10−2 3 0.348 1.009 NS 0.924 NS 1.102 NS 0
gyrB 37 505 1 0.027 0 0.000 0.000 0 0.000 NP NP NP NP
rpoD 37 674 5 0.135 6 0.890 0.353 × 10−2 6 0.701 1.777 NS 0.386 NS 0.949 NS 0
a

Number of haplotypes/number of strains.

b

Fifty-seven strains isolated in this study and the reference strain ICMP 11055, isolated in southern Iran in 1983.

c

NS, not significant.

d

NP, no polymorphism.

e

The dnaK and rpoD gene sequences in strains ICMP 5752 and CIX127, respectively, were excluded from the analyses due to unsatisfactory sequence quality.