TABLE 2.
Region, X. translucens pathovar | Gene | No. of |
Haplotype frequencya | Total no. of segregating sites | % of polymorphic sites | Nucleotide diversity (π) | No. of mutations (η) | Haplotype (gene) diversity | Value for indicated neutrality test |
Minimum no. of recombination events | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Strains | Nucleotides | Haplotypes | Tajima’s D | Fu and Li’s D* | Fu and Li’s F* | |||||||||
Iran | ||||||||||||||
undulosa | dnaK | 58b | 762 | 8 | 0.137 | 5 | 0.656 | 0.118 × 10−2 | 5 | 0.655 | −0.391 NSc | 1.083 NS | 0.724 NS | 2 |
fyuA | 58 | 522 | 1 | 0.017 | 0 | 0.000 | 0.000 | 0 | 0.000 | NPd | NP | NP | 0 | |
gyrB | 58 | 505 | 4 | 0.068 | 3 | 0.594 | 0.100 × 10−2 | 3 | 0.456 | −0.442 NS | −1.782 NS | −1.602 NS | 0 | |
rpoD | 58 | 674 | 7 | 0.120 | 6 | 0.890 | 0.123 × 10−2 | 7 | 0.257 | −1.165 NS | −0.369 NS | −0.737 NS | 2 | |
translucens | dnaK | 8 | 762 | 7 | 0.875 | 4 | 0.524 | 0.230 × 10−2 | 4 | 0.964 | 0.586 NS | 0.568 NS | 0.629 NS | 1 |
fyuA | 8 | 522 | 2 | 0.250 | 3 | 0.574 | 0.308 × 10−2 | 3 | 0.536 | 1.600 NS | 1.233 NS | 1.445 NS | 0 | |
gyrB | 8 | 505 | 3 | 0.375 | 2 | 0.396 | 0.099 × 10−2 | 2 | 0.464 | −1.310 NS | −1.409 NS | −1.513 NS | 0 | |
rpoD | 8 | 674 | 4 | 0.500 | 7 | 1.038 | 0.540 × 10−2 | 7 | 0.786 | 1.662 NS | 0.973 NS | 1.252 NS | 0 | |
Non-Iran | ||||||||||||||
undulosa | dnaK | 75 | 762 | 8 | 0.106 | 8 | 1.049 | 0.129 × 10−2 | 8 | 0.598 | −1.011 NS | 0.512 NS | 0.004 NS | 1 |
fyuA | 75 | 522 | 2 | 0.026 | 1 | 0.191 | 0.005 × 10−2 | 1 | 0.027 | −1.062 NS | −1.947 NS | −1.958 NS | 0 | |
gyrB | 75 | 505 | 2 | 0.026 | 1 | 0.198 | 0.010 × 10−2 | 1 | 0.053 | −0.904 NS | 0.513 NS | 0.110 NS | 0 | |
rpoD | 74e | 674 | 5 | 0.067 | 5 | 0.741 | 0.031 × 10−2 | 5 | 0.155 | −1.780 NS | −3.024e (P < 0.05) | −3.084e (P < 0.05) | 0 | |
translucens | dnaK | 36e | 762 | 2 | 0.055 | 1 | 0.131 | 0.042 × 10−2 | 1 | 0.322 | 0.495 NS | 0.574 NS | 0.636 NS | 0 |
fyuA | 37 | 522 | 2 | 0.054 | 3 | 0.574 | 0.200 × 10−2 | 3 | 0.348 | 1.009 NS | 0.924 NS | 1.102 NS | 0 | |
gyrB | 37 | 505 | 1 | 0.027 | 0 | 0.000 | 0.000 | 0 | 0.000 | NP | NP | NP | NP | |
rpoD | 37 | 674 | 5 | 0.135 | 6 | 0.890 | 0.353 × 10−2 | 6 | 0.701 | 1.777 NS | 0.386 NS | 0.949 NS | 0 |
Number of haplotypes/number of strains.
Fifty-seven strains isolated in this study and the reference strain ICMP 11055, isolated in southern Iran in 1983.
NS, not significant.
NP, no polymorphism.
The dnaK and rpoD gene sequences in strains ICMP 5752 and CIX127, respectively, were excluded from the analyses due to unsatisfactory sequence quality.