TABLE 3.
Primer pairs used in this study
| Primer name | Nucleotide sequence (5′–3′) | Size of amplicon (bp) | Annealing temp (°C) | Target region | Reference |
|---|---|---|---|---|---|
| T1 | CCGCCATAGGGCGGAGCACCCCGAT | 139 | 53 | 16S-23S rRNA | 7 |
| T2 | GCAGGTGCGACGTTTGCAGAGGGATCTTCTGCAAA | ||||
| XrpoD1F | TGGAACAGGGCTATCTGACC | 674 | 54 | rpoD | 37 |
| XrpoD1R | CATTCYAGGTTGGTCTGRTT | ||||
| dnaK-F | TCCTAAGCACCTCAACATCAAG | 762 | 59 | dnaK | 6 |
| dnaK-R | CTTCTTGTCGTCCTTGACCTC | ||||
| fyuA-F | CTCGCAGAACGGCCTGTA | 522 | 61 | fyuA | 6 |
| fyuA-R | GTAGCCGGGCATCTTCAACT | ||||
| XgyrPCR2F | AAGCAGGGCAAGAGCGAGCTGTA | 700 | 54 | gyrB | 38 |
| X.gyrrsp1 | CAAGGTGCTGAAGATCTGGTC |