TABLE 1.
Oligonucleotides used in this studya
| Primer | Sequence (5′–3′)b | Sitec |
|---|---|---|
| 0427-F | CATCCACCATATGATCATTTCCATAGCAGC | NdeI |
| pSeSD-0427-R | GACGGTCGACTTAGCCGAAAGTAGTCTTC | SalI |
| pK-0427-F (TG-arm) | CTCAGTCGACATGCTGCTTTTAGGAGGATA | SalI |
| pK-0427-R (TG-arm) | AACCACGCGTTTAGCCGAAAGTAGTCTTCA | MluI |
| pK-0427-F (L-arm) | CACGCCATGGTCGAGAACAACAGTAATGT | NcoI |
| pK-0427-R (L-arm) | GGAGCTCGAGGAATAGTAAATCCTTTCATC | XhoI |
| pK-0427-F (R-arm) | GCTTCTCGAGCTACTTTCGGCTAAGCTT | XhoI |
| pK-0427-R (R-arm) | GAATGCATGCGCAATGCAGAGCCTGCAGTA | SphI |
| Flank-0427-F | AAATTCGGACTTCTTACCACCATAGG | |
| Flank-0427-R | GTTGAAAGGGAATTTACGAGTGATCC | |
| CgLOG-F | GCGCCATATGACTTCGCTTTTCGACGCCCC | NdeI |
| CgLOG-R | GCGCGTCGACCCATTTTGGTGCTGGTGGAGTCC | SalI |
| 0427-R86A-F | TGTGAGTTTAGATGCGCATCCGTAATCCTTGT | |
| 0427-R86A-R | ACAAGGATTACGGATGCGCATCTAAACTCACA | |
| 0427-G101A-F | ATACTAGTTTCATTGGCAGGAGGTGTTGGAACGG | |
| 0427-G101A-R | CCGTTCCAACACCTCCTGCCAATGAAACTAGTAT | |
| 0427-G102A-F | CTAGTTTCATTGGGAGCAGGTGTTGGAACGGA | |
| 0427-G102A-R | TCCGTTCCAACACCTGCTCCCAATGAAACTAG | |
| 0427-G103A-F | GTTTCATTGGGAGGAGCTGTTGGAACGGAAA | |
| 0427-G103A-R | TTTCCGTTCCAACAGCTCCTCCCAATGAAAC | |
| 0427-G105A-F | ATTGGGAGGAGGTGTTGCAACGGAAATAGAAAT | |
| 0427-G105A-R | ATTTCTATTTCCGTTGCAACACCTCCTCCCAAT | |
| 0427-T106A-F | TGGGAGGAGGTGTTGGAGCGGAAATAGAAATAATG | |
| 0427-T106A-R | CATTATTTCTATTTCCGCTCCAACACCTCCTCCCA | |
| 0427-E109A-F | GGAACGGAAATAGCAATAATGATTGCTTACGC | |
| 0427-E109A-R | GCGTAAGCAATCATTATTGCTATTTCCGTTCC |
a
Abbreviations: TG-arm, target gene arm; L-arm, left sequence to target gene; R-arm, right sequence to target gene; F, forward; R, reverse.
b
Underlined sequences represent restriction enzyme cutting sites.
c
Restriction enzyme.