TABLE 2.
Strains and plasmids used and constructed in this study
| Strain | Characteristics/usage | Source or reference |
|---|---|---|
| S. islandicus REY15A | Wild type | Contursi et al. (41) |
| S. islandicus REY15A (E233S) | ΔpyrEF-lacS | Deng et al. (31) |
| S. islandicus Δsire_0427 | ΔpyrEF-lacS Δsire_0427 | This study |
| E. coli DH5α | Plasmid amplification | Laboratory strain |
| E. coli BL-21(DE3) codon plus-RIL | Protein expression | Laboratory strain |
| E. coli/pET22b-SiRe_0427-C-His | Wild-type SiRe_0427 | This study |
| E. coli/pET22b-SiRe_0427R86A-C-His | R86A (catalytic mutant) | This study |
| E. coli/pET22b-SiRe_0427G101A-C-His | G101A (substrate binding mutant) | This study |
| E. coli/pET22b-SiRe_0427G102A-C-His | G102A (substrate binding mutant) | This study |
| E. coli/pET22b-SiRe_0427G103A-C-His | G103A (activity similar to that of the wild type) | This study |
| E. coli/pET22b-SiRe_0427G105A-C-His | G105A (substrate binding mutant) | This study |
| E. coli/pET22b-SiRe_0427T106A-C-His | T106A (substrate binding mutant) | This study |
| E. coli/pET22b-SiRe_0427E109A-C-His | E109A (catalytic mutant) | This study |