FIG 1.

Kinetics of cell infection by T. gondii sporocysts in simple matrix assessed by sporocyst-CC-qPCR assay. Following 2 h (A) or 1 day (B) of contact between the sporocysts and Vero cells (D0), the cells were cultivated for 2 (D2), 3 (D3), or 6 days (D6). Sporocysts were obtained after TissueLyser agitation (30 s, 33 Hz) from viable and heat-inactivated oocysts (5 min at 99°C). Error bars indicate standard deviation (2 < n < 5 independent experiments). Negative control corresponds to Vero cells without sporocysts. *, P < 0.05.