TABLE 1.
Limit of detection of the T. gondii sporocyst-CC-qPCR assay in simple matrixa
| Oocyst quantity | D0 |
D3 |
||
|---|---|---|---|---|
| Cq (mean ± SD) | No. positive exptsb/total no. | Cq (mean ± SD) | No. positive exptsb/total no. | |
| 10,000 | 25.36 ± 0.59 | 3/3 | 20.40 ± 1.17c | 3/3 |
| 1,000 | 29.37 ± 0.27 | 3/3 | 24.06 ± 0.09c | 3/3 |
| 100 | 32.71 ± 0.41 | 3/3 | 27.38 ± 0.75c | 3/3 |
| 10 | 33.33 ± 1.08 | 3/3 | 28.01 ± 2.07c | 3/3 |
a
A serial dilution ranging from 10 to 10,000 T. gondii oocysts was used to obtain sporocysts resuspended in culture medium and then deposited on Vero cells (80% confluence) incubated at 37°C. qPCR specific to T. gondii was performed from the cell pellet DNA extract after a contact time between sporozoites and cells of 1 day (D0) and after culture of the cells for 3 days (D3) (n = 3 independent experiments).
b
Positive signal by qPCR in well culture.
c
Significantly different from D0 (P < 0.05).