TABLE 2.

Limit of detection of the sporocyst-CC-qPCR assay of the sporocyst-CC-qPCR assay in D. polymorpha and M. edulis^a

Parameter	Oocyst quantity	Cq (mean ± SD)
		D. polymorpha	M. edulis
D0	10,000	25.73 ± 0.42	27.34 ± 1.73
D3	50,000	20.25 ± 0.36	18.13 ± 0.21
	10,000	21.83 ± 0.54	18.82 ± 0.41
	5,000	22.43 ± 0.55	19 ± 0.34
	1,000	23.85 ± 0.13	19.49 ± 0.64
	500	23.77 ± 0.44	20.15 ± 0.16
	100	24.42 ± 0.17	19.94 ± 0.69
	50	25.08 ± 1.23	19.72 ± 0.14
	10	24.1 ± 0.20	19.44 ± 0.36
Equation		y = −1.14x + 26.47	y = −0.39x + 20.47
r²		0.81	0.57

^aOocysts (10 to 10⁴) were spiked to tissues from one mussel. Then, the tissues were processed to recover oocysts and to release sporocysts before infecting cells (see Materials and Methods). qPCR was performed at D0 (i.e., after 3 h of contact between sporocysts and cells) and at D3 (i.e., after 3 days of cell culture) (n = 3 independent experiments).