TABLE 2.
Physiological parameters of S. cerevisiae CEN.PK113-7D cultured in aerobic N- and P-limited slow-growth steady-state chemostats and near-zero-growth pseudo-steady-state retentostatsa
| Culture condition | Viability (%) | RQb | Yx/sc | Yp/sd | Carbon recovery (%) | Reduction recovery (%) | Residual nutrient concn |
||
|---|---|---|---|---|---|---|---|---|---|
| Glucose (g/liter) | NH4+ (mM) | PO43− (mM) | |||||||
| N limited at SG | 93 ± 0 | 3.1 ± 0.0 | 0.059 ± 0.000 | 0.32 ± 0.12 | 95 ± 1 | 95 ± 1 | 16.5 ± 0.2 | BD | 18.4 ± 0.9 |
| P limited at SG | 91 ± 0 | 3.1 ± 0.1 | 0.052 ± 0.000 | 0.36 ± 0.00 | 99 ± 6 | 99 ± 3 | 18.2 ± 0.7 | 40.5 ± 2.0 | BD |
| N limited at NZG | 80 ± 0 | 2.1 ± 0.1 | — | 0.33 ± 0.02 | 101 ± 2 | 98 ± 3 | 15.0 ± 2.0 | BD | 18.2 ± 0.9 |
| P limited at NZG | 90 ± 0 | 1.7 ± 0.0 | — | 0.28 ± 0.03 | 98 ± 2 | 99 ± 1 | 10.3 ± 0.2 | 53.5 ± 2.7 | BD |
a
Data represent averages, with their standard errors, calculated from multiple measurements obtained from duplicate experiments. Slow growth (SG), μ = 0.025 h−1; near-zero growth (NZG), μ < 0.002 h−1; BD, below detection limit of assay; —, not calculated.
b
RQ, respiratory quotient (qCO2/qO2).
c
Yx/s, yield of biomass (g biomass/[g glucose consumed]).
d
Yp/s, yield of ethanol (g ethanol/[g glucose consumed]).