TABLE 3.
Biomass elemental compositions of S. cerevisiae CEN.PK113-7D cultured in aerobic N- and P-limited slow-growth steady-state chemostats and near-zero-growth pseudo-steady-state retentostatsa
| Culture condition | μ (h−1) | fC (%)b | fH (%) | fN (%) | fO (%) | fP (%) | fS (%) | Sum (%) | C-molc wt (g) |
|---|---|---|---|---|---|---|---|---|---|
| N limited | 0.025 | 47.0 ± 0.0 | 7.2 ± 0.0 | 3.5 ± 0.1 | 39.5 ± 0.4 | 1.3 ± 0.0 | 0.16 ± 0.01 | 98 ± 0 | 25.6 ± 0.0 |
| P limited | 0.025 | 44.0 ± 0.2 | 7.0 ± 0.1 | 5.3 ± 0.0 | 36.9 ± 0.3 | 0.50 ± 0.01 | 0.39 ± 0.03 | 94 ± 0 | 24.3 ± 0.3 |
| N limited | <0.002 | 49.5 ± 0.4 | 7.6 ± 0.0 | 2.5 ± 0.0 | 39.5 ± 1.1 | 1.1 ± 0.0 | 0.11 ± 0.01 | 100 ± 1 | 27.3 ± 0.2 |
| P limited | <0.002 | 47.3 ± 0.1 | 7.2 ± 0.0 | 4.6 ± 0.1 | 37.4 ± 0.0 | 0.29 ± 0.00 | 0.27 ± 0.01 | 97 ± 0 | 25.4 ± 0.1 |
| C limited | 0.022 | 45.6 | 6.8 | 6.6 | 37.3 | 1.0 | 0.22 | 97 | 26.4 |
a
Data represent averages, with standard errors, of measurements from duplicate cultures and are compared with published values from aerobic glucose-limited (C-limited) chemostat culture of the same strain (49). Slow growth, μ = 0.025 h−1; near-zero growth, μ < 0.002 h−1.
b
Balanced elemental mass fractions f in %.
c
The amount of biomass (g) containing 1 mol of carbon.