FIG 2.

X. perforans strains collected in Alabama carry a plasmid nearly identical to one found in X. cynarae pv. gardneri, which harbors the transcription activation-like effector AvrHah1, and are pathogenic to pepper. (A) Alignment of contigs assembled with plasmidSPAdes software from X. perforans strain AL65 and the X. cynarae pv. gardneri (Xcg) plasmid JS749-3.2. The inner ring shows the GC content of the plasmid, and the annotations of coding sequences (CDS) (shown in black) are provided where available. (B) Bacterial spot symptoms produced by two strains from SC6, with and without AvrHah1, shown 4 days postinoculation (DPI). Leaves of the susceptible pepper cultivar ECW were infiltrated with a needleless syringe using a bacterial suspension adjusted to 10⁴ CFU ml⁻¹. The individual strains used for inoculation are shown in parentheses. (C) Compatible and incompatible interactions on pepper cultivar ECW30R with Bs3 resistance, shown 2 days postinoculation with a bacterial suspension raised to 10⁸ CFU ml⁻¹. Hypersensitive resistance mediated by the recognition of AvrHah1 in strain AL65 is shown on the top left portion of the leaf. Pepper strains ALS7B and ALS7E, from SC6 and SC3, are shown on the bottom left and bottom right portions of the leaf, respectively.