FIG 4.

HPAEC-PAD and MALDI-TOF MS analyses of GH95 hydrolysis products from fucosylated XyGOs. A mixture of fucosylated XyGOs (XXXG, XXFG, XLXG, and XLFG) was produced by the action of BoGH5A (24) on lettXyG, followed by incubation with 2 μM recombinant BuGH95, BfGH95, or DgGH95 at 37°C for 16 h in 50 mM sodium citrate buffer, pH 6.0. In each case, XLFG and XXFG were converted into XLLG and XXLG, as confirmed by comparison with the XyGOs produced by BoGH5 on tamXyG. The observed molecular masses of the peaks in MALDI-TOF MS correspond to the [M + Na]⁺ ions of XXXG (calculated: 1,085.9), XLXG/XXLG (calculated: 1,248.05), XXFG (calculated: 1,394.21), XLLG (calculated: 1,410.19), and XLFG (calculated: 1,556.35).