TABLE 1.
Michaelis-Menten kinetic constants of BoGH5A, BuGH5, BcGH5, BfGH5, and DgGH5 against tamXyG
| Bacterial strain | Enzyme | Substrate | kcat (s−1) | Km (mg · ml−1) | kcat/Km (s−1 · mg−1 · ml) | Assay |
|---|---|---|---|---|---|---|
| B. ovatus ATCC 8483 | BoGH5 | tamXyG | 8.9 ± 0.2a | 0.046 ± 0.006 | 190 | BCA |
| B. cellulosilyticus DSM 14838 | BcGH5 | tamXyG | 31.0 ± 1.0 | 0.036 ± 0.003 | 862 | BCA |
| B. uniformis ATCC 8492 | BuGH5 | tamXyG | 19.6 ± 0.8 | 0.043 ± 0.007 | 456 | BCA |
| B. fluxus YIT 12057 | BfGH5 | tamXyG | 50.1 ± 3.0 | 0.17 ± 0.02 | 295 | BCA |
| D. gadei ATCC DAA-286 | DgGH5 | tamXyG | 19.0 ± 1.0 | 0.039 ± 0.009 | 487 | BCA |
a
All error values correspond to errors in fitting the Michaelis-Menten equation to the data.