TABLE 2.
Michaelis-Menten kinetic constants of BuGH95, BfGH95, and DgGH95 against 2-chloro-4-nitrophenyl α-l-fucopyranoside
| Bacterial strain | Enzyme | Substrate | kcat (s−1) | Km (mg · ml−1) | kcat/Km (s−1 · mg−1 · ml) | Assay |
|---|---|---|---|---|---|---|
| B. uniformis ATCC 8492 | BuGH95 | l-Fuc-α-CNP | 2.0 ± 0.1a | 1.7 ± 0.1 | 1.2 | CNP |
| B. fluxus YIT 12057 | BfGH95 | l-Fuc-α-CNP | 1.9 ± 0.1 | 3.8 ± 0.3 | 0.49 | CNP |
| D. gadei ATCC DAA-286 | DgGH95 | l-Fuc-α-CNP | 3.4 ± 0.2 | 4.7 ± 0.4 | 0.73 | CNP |
a
All error values correspond to errors in fitting the Michaelis-Menten equation to the data.